Established from the malignant tumor of a 49-year-old woman with well-differentiated oxyphilic papillary thyroid carcinoma (PT2, G1, No, Mo); cells were described to be tumorigenic in nude mice; however, DNA fingerprinting analysis at the Creative Bioarray showed cross-contamination with cell line OVCAR-3; OVCAR-3 line was established from the malignant ascites of a patient with progressive adenocarcinoma of the ovary
Human hypotriploid karyotype with 8% polyploidy - 59(56-61)<3n>XX, -X, +1, -2, -4, -6, -8, -9, -13, -13, -14, -15, -15, -17, -17, -18, -19, -21, -22, +7-9mar, +0-3 dm, add(1)(p3?5), add(1)(p2?1), add(1)(q11), der(3;15)(p10;q10), add(3)(p14), del(5)(q23),
epithelial-like adherent cells with large vacuoles growing as monolayers
Mycoplasma: contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endot
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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