Chicken Red Blood Cells

Cat.No.: CSC-C3094

Species: Chicken

Source: Blood

Cell Type: Red Blood Cell

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Cat.No.
CSC-C3094
Description
The whole blood is aseptically collected and prepared to order from our colony of animals. These washed cell suspensions are useful for the titration of complement, absorption procedures, testing for agglutinins, and for the preparation of stroma as particulate reagents.
Species
Chicken
Source
Blood
Cell Type
Red Blood Cell
Disease
Normal
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Chicken red blood cells (cRBCs) represent a unique population of nucleated erythrocytes whose precursor cells arise mainly from the bone marrow. In adult birds, mature erythrocytes are released into the peripheral blood, and represent the most abundant cell type. Morphologically, cRBCs are oval, biconcave cells that are 9-12 µm in diameter. The nucleated cells have centrally-located nucleus, making them easily distinguishable from mammalian anucleated erythrocytes. Hemoglobin within their cytoplasm stains cRBCs yellow‑green.

In vivo, cRBCs function as the primary oxygen transporter. The glycoconjugates on their surfaces allow for hemagglutination to avian viruses. For this reason, cRBCs are widely used as reagents for hemagglutination and hemagglutination‑inhibition assays for detecting antibodies to influenza, Newcastle disease virus, and other viruses of poultry. In addition, cRBCs have recently been shown to up‑regulate pro‑inflammatory cytokines (e.g., TNF‑α, IFN‑γ, and IL‑1β) in response to antimicrobial peptides (e.g., AvBD11). This previously-unknown function of cRBCs could be exploited for future studies of innate immune responses. Chicken red blood cells can also be used for in vitro serological assays, testing vaccine preparations for efficacy, and determining the signals elicited by immunological pathways, as well as in genomic studies to understand avian chromatin architecture.

Converting of the 2D Graphene to its 3D By Chicken Red Blood Cells as Sheets Separator for Construction Supercapacitor Electrode

Traditional 3D graphene lacks cost-effective, green spacers and heteroatom dopants. Jamshidi et al. introduced chicken red blood cells as both porogen and N/S source to fabricate porous 3D graphene for high-performance supercapacitors.

Chicken red blood cells (RBCs) were used as raw materials for synthesizing graphene-based composites. GO-RBCs hybrids were formed by stirring a mixture containing a suspension of GO and RBCs for 5 hours. Subsequently, a freeze-drying method was employed to produce the macro-porous hybrids (GO-RBCs-F). Finally, to enhance the micro-porosity and improve the conductivity of the composites, the material was pyrolyzed under a nitrogen atmosphere, resulting in the product denoted as GO-RBCs-F-P. Before using the freeze casting method to produce the macro-porous hybrid, the interaction between RBCs and GO was investigated by examining the zeta potentials of the reactants and products. Since the pH of graphene oxide suspension is around 3.5, the zeta potential investigation was conducted at this pH. As shown in Figure 1A, the zeta potential of the RBCs solution at pH 3.5 is about 15 mV. The zeta potential of GO (Fig. 1B) was -28 mV, which is consistent with other reports on GO. After mixing the RBCs solution with GO, the zeta potential of the GO-RBCs mixture changed to -18 mV, indicating successful interaction between GO and RBCs (Fig. 1C). In this step, as expected, the surface of the GO sheets was covered with RBCs or hemoglobin, facilitating further separation of the GO sheets.

The steps of the preparation of GO-RBCs-F-P composite.

Fig. 1. The steps of the preparation of GO-RBCs-F-P composite (Jamshidi F, Gholivand M B, et al., 2022).

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