MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant murine MEC is a 12.6 kDa protein containing 111 amino acid residues.
Mouse Ccl28 expressed in E.coli
CAT# CSC-CTK0786-20 (20 μg); CAT# CSC-CTK0786-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Determined by its ability to chemoattract murine lymphocytes using a concentration range of 1- 10 ng/ml.
Less than 1 EU/μg.
The protein was lyophilized from 10 mM NaCitrate, pH 4.0.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu MEC should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
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