TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic death domain, which can activate the cells apoptotic machinery. These receptors are activated by binding to wither membrane anchored or soluble TRAIL/Apo2L. Recombinant human soluble TRAIL Receptor-2/DR5 is a 14.9 kDa protein (133 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain.
Human TNFRSF10B expressed in E.coli
CAT# CSC-CTK0336-50 (50 μg); CAT# CSC-CTK0336-250 (250 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Determined by its inhibitory effect of IL-8 production in human PBMC by human sTRAIL/Apo2L. 50% inhibition (100 ng/ml of s TRAIL/Apo2L) was reached using a concentration of 1000 ng/ml of sTRAIL Receptor-2.
Less than 1 EU/μg.
The sterile-filtered solution was lyophilized with no additives.
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted sTRAIL Receptor-2 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
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