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ActoFactor™ Recombinant Human Tumor necrosis factor (ligand) superfamily, member 13b (7.7 kDa)

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Cat.No.
CSC-CTK0357
Description
BAFF Receptor (BAFF-R), a member of the TNFR superfamily, is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF-R is a type III transmembrane protein that binds with high specificity to BAFF (TNFSF13B). BAFF-R/BAFF signaling plays a critical role in B cell survival and maturation. Recombinant human BAFFR is a 76 amino acid polypeptide (7.7 kDa) corresponding to the extracellular portion of the full BAFF-R protein.
Organism
Human
Product Overview
Human TNFSF13B expressed in E.coli
Molecular Mass
7.7 kDa
Size
CAT# CSC-CTK0357-50 (50 μg); CAT# CSC-CTK0357-250 (250 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to block BAFF induced mouse splenocyte survival. The expected ED50 for this effect is 2.0-4.0 μg/ml in the presence of 1.0 μg/ml of human soluble BAFF.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered through a 0.2 micron filter and lyophilized from 0.5x PBS (pH 7.2).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BAFF-R should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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