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ActoFactor™ Recombinant Human Tumor necrosis factor (ligand) superfamily, member 13b (17 kDa)

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Cat.No.
CSC-CTK0355
Description
BAFF, a member of the TNF family of ligands, is expressed in T cells, macrophages, monocytes and dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors TACI, BCMA and BAFF-R. Using specific mAbs, BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice, and antagonist mAbs to BAFF-R blocked BAFF-mediated costimulation of anti- micro responses. The other BAFF receptors showed a much more restricted expression pattern, suggestive of specialized roles. Recombinant human B lymphocyte stimulator produced in E. coli is a single, non-glycosylated polypeptide chain of 153 amino acids and a molecular mass of 17,007 Dalton.
Organism
Human
Product Overview
Human TNFSF13B expressed in E.coli
Molecular Mass
17 kDa
Size
CAT# CSC-CTK0355-20 (20 μg); CAT# CSC-CTK0355-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Recombinant B Lymphocyte Stimulator is fully biologically active when compared to a standard. The activity is determined by a mouse splenocyte survival assay. The ED50 for this effect is 0.5 - 2.0 μg/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
White (freeze-dried) powder. Lyophilized from a sterile-filtered protein solution in PBS (pH 7.0).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu BAFF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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