Slit2 is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and can also act as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. Slit2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. Slit2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant human Slit2-N is a 1088 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0 kDa by SDS-PAGE analysis under reducing conditions.
Human SLIT2 expressed in HEK293 cells
CAT# CSC-CTK0620-25 (25 μg); CAT# CSC-CTK0620-125 (125 μg)
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Determined by ist ability to inhibit MC3T3/E1 osteoblast cell differentiation.
Less than 1 EU/μg.
Lyophilized from a sterile-filtered solution with 20 mM Tris + 200 mM L-Arginine (pH 8.8).
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with sterile 20 mM Tris (pH 8.8), 150 mM NaCl to a concentration of 0.1 - 1.0 mg/ml (dissolves slowly). For further dilution, a carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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