Vascular Endothelial Growth Factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, it may play a role in stimulating vasolidation via nitric oxide-dependent pathways. Alternative splicing of the mRNA for VEGF-A results in several protein isoforms. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. Recombinant human VEGF produced in HEK cells is a glycosylated dimeric polypeptide chain of 165 amino acids and a molecular mass of 40,000 Dalton.
Human NRP1 expressed in HEK293 (Human Embryonic Kidney cell line)
CAT# CSC-CTK0402-10 (10 μg); CAT# CSC-CTK0402-50 (50 μg)
HEK293 (Human Embryonic Kidney cell line)
Greater than 95% as determined by SDS-PAGE analysis.
VEGF-165 is fully biologically active when compared to standards. Determined by the dose- dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-6.0 ng/ml, corresponding to a specific activity of 1 x 10^6 Units/mg to 1.7 x 10^5 Units/mg.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) so lution with no additives.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu VEGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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