Leukemia inhibitory factor (LIF) is a pleiotropic lymphoid factor belonging to the interleukin-6 family of cytokines. It was initially described as a factor inhibiting the proliferation of myeloid leukemia cells and inducing their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis and roles in cholinergic neuron differentiation, hematopoeisis, inflammation and bone metabolism. LIF inhibits the differentiation of embryonic stem cells, it is therefore important in ES cell culture. Human and mouse LIF share 78% amino acid homology, the human version is equally active on human and mouse cells but murine LIF is not active on human cells. Recombinant human LIF contains 180 amino acids and a 16 a.a. histidine-based tag for a total length of 196 a.a. and has a predicted molecular mass of 21.8 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25-30 kDa in SDS-PAGE. Recombinant human LIF is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E. coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1 beta induction.
Human LIF expressed in Endosperm tissue of barley grain (Hordeum vulgare)
CAT# CSC-CTK0108-10 (10 μg); CAT# CSC-CTK0108-50 (50 μg)
Endosperm tissue of barley grain (Hordeum vulgare)
Greater than 95% by SDS-PAGE gel analysis.
The bioactivity of plant-derived human LIF was determined by its ability to induce proliferation of TF-1cells in a dose dependent manner. The ED50 for this batch of recombinant LIF is 0.7 ng/ml, corresponding to 2 x 10^6 units/mg specific activity. Optimal concentration should be determined for specific applications and cell lines.
Less than 0.05 EU/μg.
Lyophlized from a sterile-filtered protein solution in PBS (pH 7.2).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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