ActoFactor™ Recombinant Human Lectin, galactoside-binding, soluble, 7

CSC-CTK0583

Galectins are a family of animal lectins with an affinity for beta-galactosides. This family has at least 14 identified members. Galectins share similarities in the CRD (the carbohydrate recognition domain). Galectins are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are involved in modulating cell-cell and cell-matrix interactions. Human Galectin-7 belongs to the prototypical Galectins containing a single CRD, which is initially identified in human epidermis as a monomer. The Galectin-7 expression is induced by tumor suppressor protein p53 and associated with apoptosis. Galectin-7 is a pro-apoptotic protein which functions intracellularlly upstream of JNK activation and mitochondrial cytochrome c release. The correlation of Galectin-7 with the UV-induced apoptosis of keratinocytes presents a critical mechanism in the maintenance of epidermal homeostasis. Human Galectin-7 is localized in both nucleus and cytoplasm. Human recombinant Galectin-7 produced in E. coli is a single, non-glycosylated polypeptide chain of 135 amino acids and a molecular mass of 15 kDa. It has been purified by proprietary chromatographic techniques.

Human

Human LGALS7 expressed in E.coli

15 kDa

CAT# CSC-CTK0583-10 (10 μg); CAT# CSC-CTK0583-50 (50 μg)

E.coli

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

rHu Galectin-7 is fully biologically active when compared to a standard. The ED50 of Galectin-7 as determined by hemagglutination test is 0.75-3 μg/ml.

Less than 1 EU/μg.

Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) protein solution in 20 mM PBS (pH 7.4).

Please centrifuge product before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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