Interleukin 33 (IL-33) is a 32 kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1 which induces helper T cells to produce type 2 cytokines and acts through the receptor IL-1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappaB and MAP kinases and induces Th2 cells to produce cytokines in vitro. In vivo, IL-33 induces expression of IL-4, IL-5, IL- 13 and leads to severe pathological changes in mucosal organs. In vitro, it can be cleaved to an N-terminal fragment of 12 kDa and an C-terminal fragment of 18 kDa by Caspase-1.|Human recombinant Interleukin 33 produced in E. coli is a single, non-glycosylated polypeptide( chain of 160 amino acids having a molecular mass of 18 kDa. It has been purified by using proprietary chromatographic techniques.
Human IL33 expressed in E.coli
CAT# CSC-CTK0275-10 (10 μg); CAT# CSC-CTK0275-50 (50 μg)
Greater than 95% as determined by SDS-PAGE and HPLC.
Sterile-filtered colorless liquid (concentration: 1 mg/ml) in 20 mM Tris (pH 8).
Please centrifuge product briefly before opening vial. The protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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