Interleukin-12 (IL-12) is a heterodimer cytokine composed of p40 and p35 subunits. It is mainly produced by macrophages, dendritic cells, and B cells. It has multiple effects on T and natural killer (NK) cells. In particular, it appears to be a major factor for the development of cellular immunity. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. The p40 subunit of IL-12 interacts with the β-subunit of IL-12R and the disulfide linked p40 homodimer specifically antagonizes the activity of bioactive IL-12 in different assay systems. Recombinant human Interleukin-12 p40 is a glycosylated polypeptide chain containing 312 amino acids (fragment 23 – 328 P29460 IL12B_HUMAN) with an amino- terminal hexahistidine tag. The recombinant protein has a predicted molecular mass of 35.5 kDa, however as a result of potential glycosylation, the molecular mass could be approximately 40k Da by SDSPAGE under reducing conditions. Human recombinant IL-12-p40 expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.
Human IL12B expressed in Nicotiana benthamiana
CAT# CSC-CTK0250-10 (10 μg); CAT# CSC-CTK0250-50 (50 μg)
Greater than 97% as determined by SDS-PAGE analysis.
rHu IL-12-p40 is fully biologically active when compared to standards. The biological activity of IL-12-p40 is determined by the dose dependent inhibition of IL-12 dependent IFN-gamma production (mouse splenocytes). Data not yet available.
Less than 0.04 EU/μg.
Lyophilized from a concentrated, sterile protein solution in 0.05 M Tris-HCl buffer (pH 7.4).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Optimal concentration should be determined for specific application and cell lines.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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