Human Interleukin 10 (IL-10) is a species cross-reactive cytokine produced by CD4+ as well as by some CD8+ T cells. In addition, human B cells, EBV-transformed lymphoblastoid cell lines and monocytes can produce IL-10 upon activation. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or
immunosuppressive effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation , Ig secretion and class II MHC expression. IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development. As an immunosuppressant, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by downregulating class II MHC expression. It inhibits IFN-g synthesis and Th1 differentiation in the mouse system. It can suppress the production of numerous cytokines including TNF-a, IL-1 and IL-6, as well as the synthesis of superoxide anion, reactive oxygen intermediates (ROI), and reactive nitrogen intermediates (RNI) by activated monocytes/macrophages. Recombinant human IL-10 containing 160 amino acid residues has a predicted molecular mass of approximately 18.6 kDa.
Human IL10 expressed in Sf21
A DNA sequence encoding the mature human IL-10 protein was expressed in Sf21 insect cells.
CAT# CSC-CTK0082-5 (5 μg); CAT# CSC-CTK0082-25 (25 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined in a cell proliferation assay using the murine mast cell line, MC/9 and the ED50 using this assay was typically 0.5 – 1.0 ng/ml.
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted IL-10 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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