Recombinant Human Interferon-gamma (rHu IFN-gamma) is a regulatory protein produced by activated NK cells and CD4+ and CD8+ T lymphocytes. IFN-gamma is capable to influence antigen presenting cells and to stimulate a number of lymphoid cell functions including the anti- microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN- gamma is a 16.9 kDa protein containing 144 amino acid residues.
Human IFNG expressed in E.coli
CAT# CSC-CTK0190-100 (100 μg); CAT# CSC-CTK0190-500 (500 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Recombinant human IFN-gamma is fully biologically active when compared to standards. The ED50, as determined in a viral resistance assay, is ≤ 0.05 ng/ml corresponding to a specific activity of Greater than 2 x 10^7 units/mg.
Less than 1 EU/μg.
Sterile-filtered (0.2 μm) and lyophilized from a concentrated (1 mg/ml) protein solution in PBS (pH 7.4).
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IFN-gamma should be stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
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