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ActoFactor™ Recombinant Human Fc fragment of IgE, low affinity II, receptor for (CD23)

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Cat.No.
CSC-CTK0302
Description
CD23 is is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is found both as a transmembrane receptor protein and in a soluble form, which is generated by proteolytic cleavage of membrane bound CD23. The predominant soluble form of CD23 (sCD23) consists of 172 amino acids corresponding to the extracellular domain of the full length precursor. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.
Organism
Human
Product Overview
Human FCER2 expressed in E.coli
Molecular Mass
19.2 kDa
Size
CAT# CSC-CTK0302-20 (20 μg); CAT# CSC-CTK0302-100 (100 μg)
Expression System
E.coli
Purity
Greater than 96% as determined by HPLC and SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile filtered through a 0.2 micron filter and lyophilized from 0.5x PBS (pH 8.0).
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored dessicated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at –20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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