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ActoFactor™ Recombinant Human Colony stimulating factor 3 (granulocyte)

  • Specification
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Granulocyte colony-stimulating factor (G-CSF) is a pleiotropic cytokine with an effect on the proliferation, differentiation and activation of granulocyte and primitive hematopoietic progenitors. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Other cell types that can secrete G-CSF include fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Two distinct cDNA clones for human G-CSF encoding 207 and 204 amino acid precursor proteins have been isolated. The recombinant G-CSF contains 175 amino acid residues and has a predicted molecular mass of approximately 18.8 kDa.
Product Overview
Human CSF3 expressed in E.coli
A DNA sequence encoding the mature human G-CSF protein generated from the 204 amino acid residue precursor G-CSF isoform1,2 was expressed in E. coli.
CAT# CSC-CTK0044-5 (5 μg); CAT# CSC-CTK0044-25 (25 μg)
Expression System
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of recombinant human G-CSF is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 10 - 60 pg/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Recombinant human G-CSF is lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid and 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitute human G-CSF at a concentration greater than 10 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized human G-CSF is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted human G-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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