A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system.CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways (endoplasmatic reticulum/Golgi system). The mechanism underlying the release of CNTF is unknown. Recombinant human CNTF produced in E. coli is a single, non-glycosylated polypeptide chain of 199 amino acids and a molecular mass of 22,706 Dalton.
Human CNTF expressed in E.coli
CAT# CSC-CTK0482-20 (20 μg); CAT# CSC-CTK0482-100 (100 μg)
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Recombinant human Ciliary Neurotrophic Factor is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependent stimulation of TF-1 cells, is less then 2 ng/ml corresponding to a specific activity of 5 x 10^5 IU/mg.
Less than 1 EU/μg.
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml) solution in water containing 5 mM sodium pho sphate buffer (pH 7.5) and 5 mM sodium chloride.
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu CNTF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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