Stromal cell-derived factor-1 (SDF-1), also known as Pre-B cell growth stimulating factor (PBSF), is a chemokine secreted by bone marrow and other stromal cells. SDF-1 is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1 was shown to be a ligand for fusin/LESTR, a CXCR4 type receptor that functions as a coreceptor for HIV-1 strains. Recently, SDF-1 was shown to induce lymphocyte homing, mature blood cell functional activation and stem cell growth inhibition. SDF-1 is also a chemoattractant for CD34+ hemopoietic progenitors. Human SDF-1a is synthesized as a precursor of 89 amino acid residues processed to the mature form by cleavage of the N-terminus.
Human CXCL12 (22-89) expressed in E.coli
A DNA sequence encoding the mature human SDF-1a protein (aa residues 22-89) was expressed in E. coli.
CAT# CSC-CTK0016-10 (10 μg); CAT# CSC-CTK0016-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by the ability to chemoattract 5 – 10 day cultured human T lymphocytes and the ED50 using this assay was typically 3 – 9 ng/ml.
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted SDF-1 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
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