USA USA: 1-631-626-9181 Europe Europe: 44-208-144-6005  


Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

Web Search    Google
Featured Products
Ordering Information

Payment methods we support:
✭ Invoice / Purchase Order
✭ Credit card

ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 12 (22-89)

  • Specification
  • Recommended Products
Stromal cell-derived factor-1 (SDF-1), also known as Pre-B cell growth stimulating factor (PBSF), is a chemokine secreted by bone marrow and other stromal cells. SDF-1 is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1 was shown to be a ligand for fusin/LESTR, a CXCR4 type receptor that functions as a coreceptor for HIV-1 strains. Recently, SDF-1 was shown to induce lymphocyte homing, mature blood cell functional activation and stem cell growth inhibition. SDF-1 is also a chemoattractant for CD34+ hemopoietic progenitors. Human SDF-1a is synthesized as a precursor of 89 amino acid residues processed to the mature form by cleavage of the N-terminus.
Product Overview
Human CXCL12 (22-89) expressed in E.coli
A DNA sequence encoding the mature human SDF-1a protein (aa residues 22-89) was expressed in E. coli.
CAT# CSC-CTK0016-10 (10 μg); CAT# CSC-CTK0016-50 (50 μg)
Expression System
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by the ability to chemoattract 5 – 10 day cultured human T lymphocytes and the ED50 using this assay was typically 3 – 9 ng/ml.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Lyophilized from a sterile-filtered solution in 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/ml of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted SDF-1 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
Please input "bioarray" as verification code.
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828

Tel: 44-208-144-6005