Monocyte chemotactic protein-3 (MCP-3) is a member of the b (CC) family of chemokines. It is produced by fibroblasts, platelets, MG63 osteosarcoma cells, mast cells and monocytes. MCP-3 is a chemoattractant for monocytes, NK cells, T cells, eosinophils, basophils, dendritic cells and neutrophils. MCP-3 binds to CCR2 and CCR3 receptors. Human MCP-3 cDNA encodes a 99 amino acid residue precursor protein containing a 23 amino acid signal peptide. Recombinant mature human MCP-3 contains 76 amino acid residues and it has an apparent molecular mass of 9 kDa.
Human CCL7 expressed in E.coli
A DNA sequence encoding the mature human MCP-3 protein was expressed in E. coli.
CAT# CSC-CTK0025-10 (10 μg); CAT# CSC-CTK0025-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined by ability to chemoattract 2 day cultured human monocytes and THP-1 cells. The ED50 for these effects were typically 0.02 - 0.08 mg/mL and 0.02 - 0.06 mg/mL, respectively.
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Lyophilized from a sterile solution in 30% acetonitrile, 0.1% TFA containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted human MCP-3/CCL7 can be stored under sterile conditions at 2°to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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