Immortalized Human Retinal Microvascular Endothelial Cells-hTERT
Cat.No.: CSC-I1903Z
Species: homo sapiens
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I1903Z
Description
Primary human retinal neuronal cells were isolated and transfected with full-length human telomerase reverse transcriptase (hTERT) expressing lentiviral particles. This cell line may be utilized as an in vitro model to study the molecular basis of several diseases of the human retina. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Species
                                homo sapiens
                            Recommended Medium
                                SuperCult? Immortalized Human Retinal Microvascular Endothelial Cell Medium (Cat No.: CM-I1903Z)
                            Freezing Medium
                                Complete medium supplemented with 10% (v/v) DMSO
                            Culture Properties
                                Adherent
                            Morphology
                                Polygonal
                            Immortalization Method
                                Human telomerase reverse transcriptase (hTERT)
                            Application
                                For Research Use Only
                            Growth Properties
                                Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
                            Shipping
                                Dry Ice.
                            Recommended Products
                                CSC-C4361X  Human Retinal Microvascular Endothelial Cells
CIK-HT013 HT? Lenti-hTERT Immortalization Kit
                            CIK-HT013 HT? Lenti-hTERT Immortalization Kit
Quality Control
                                Real Time PCR was used to quantify hTERT gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
                            Storage and Shipping
                                Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
                            Note: Never can cells be kept at -20 °C.
Citation Guidance
                        
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