Immortalized Human Neonatal Fibroblasts (MRC-5)
Cat.No.: CSC-I2297Z
Species: homo sapiens
Morphology: Polygonal
Culture Properties: Adherent
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MRC-5 is a well-characterized, diploid human fetal lung fibroblast cell line derived from a 14-week-old male fetus. It is not immortalized and not neonatal; it retains a finite in vitro lifespan and normal cell-cycle checkpoints, making it a physiologically relevant, non-transformed model. MRC-5 expresses fibroblast markers such as vimentin, collagen I/III, and fibronectin, and is highly permissive to many human and animal viruses, including varicella-zoster, poliovirus, hepatitis A, rabies, and cytomegalovirus.
Key advantages:
- Regulatory acceptance: MRC-5 is approved for human vaccine production (e.g., varicella, hepatitis A, polio, rabies) due to its stable diploid karyotype, genetic fidelity, and low tumorigenicity.
- Reproducibility: Extensive historical data, standardized culture protocols, and broad availability support consistent, comparable results.
- Broad viral susceptibility: Ideal for virus isolation, propagation, titration, neutralization assays, and vaccine development.
- Safety testing: Widely used for cytotoxicity, genotoxicity, and biocompatibility assessment.
- Senescence research: Its finite lifespan enables studies of replicative senescence and ageing.
- Genetic tractability: Amenable to transfection, siRNA, and CRISPR for mechanistic studies.
Overall, MRC-5 offers a robust, regulatory-compliant, and human-relevant platform for virology, vaccine development, safety testing, and translational research.
Development and Characterization of MRC-5 and Vero Cell-Adapted Enterovirus D68 Strains for Vaccine Production
Enterovirus D68 (EV-D68) is a pathogen that causes respiratory and neurological diseases. Currently, there are no licensed vaccines for EV-D68. Here, we adapted EV-D68 to MRC-5 and Vero cells, which are widely used in vaccine manufacturing, to develop EV-D68 strains applicable for vaccine production. We successfully isolated MRC-5 cell-adapted strains by serial passaging in MRC-5 cells. Although efforts to isolate Vero cell-adapted strains through serial passaging of EV-D68 in Vero cells were unsuccessful, we isolated Vero cell-adapted strains by serial passaging of MRC-5 cell-adapted strains in Vero cells. Inactivated whole-virion vaccines were prepared from vaccine-manufacturing cell-adapted strains and mice were immunized with these vaccines. We found that in some cases, the parental and cell-adapted strains induced similar levels of protective immunity against EV-D68, whereas in other cases, the cell-adapted strains were significantly less effective than the parental strains. These data provide valuable information for EV-D68 vaccine production.
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- Adipose Tissue-Derived Stem Cells
- Human Neurons
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- Human Adult Stem Cells
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