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TI-4
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Cat.No.
CSC-C6181X
Description
Described as secreting a mouse monoclonal antibody (IgG1) reacting with rat cardiac fibers in immunofluorescence and with rat adult and fetal cardiac troponin I in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified troponin complex from bovine adult heart
Species
Mouse
Source
hybridoma (anti-rat fiber/troponin I)
Recommended Medium
80% DMEM ) + 10-20% horse serum.
Culture Properties
suspension
Morphology
cells grow in suspension as single cells or in small clumps
Karyotype
murine karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 4-6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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