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CF-1D12

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Cat.No.
CSC-C6160X
Description
Described as secreting a mouse monoclonal antibody (IgG3) CD52; the cell line was established in 1989 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with IL-2-driven T cell lines from patients with high numbers of gamma-delta T cells in the peripheral blood
Species
Mouse
Source
hybridoma (CD52)
Recommended Medium
90% DMEM + 10% h.i.FBS + HFCS ("Hybridoma Fusion and Cloning Supplement")
Culture Properties
suspension
Morphology
round, single cells growing in suspension and some slightly adherent
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG3 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CM-1010X

For research use only. Not for any other purpose.

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