SK-MEL-30

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Cat.No.

CSC-C0302

Description

Derived from tumor tissue (subcutis metastasis) of a 67-year-old Caucasian man with malignant melanoma in 1975

Species

Human

Source

melanoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent, epithelial-like cells growing as monolayers

Karyotype

Human hypertriploid karyotype with 3.5% polyploidy - 80(69-83)<3n>XXY-Y/XXYY, +1, +1, +2, +2, +3, +7, +10, -11, +12, +17, +18, +20, -21, +2mar, inv(6)(p11;q25), der(7)t(7;14)(p22;q22), der(9)t(9;?21)(p11;q11)x2, 14p+, der(15)t(15;?)(p12;?)x2, der(16)t(16;

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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