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SC-75

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Cat.No.
CSC-C6157X
Description
Described as secreting a mouse monoclonal antibody (IgM) reacting with all adult rat type 2 fibers in immunofluorescence and with all adult rat type 2 MHCs (myosin heavy chain) in Western blotting; recognizing also rat neonatal fibers in immunofluorescence and rat neonatal MHCs in Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle
Organism
Mouse
Karyotype
Murine hypertetraploid karyotype with 5% polyploidy - 86-96<4n> - 1-3 centric fusion markers and 1-2 dmin present in most cells
Source
hybridoma
Culture Properties
suspension
Morphology
single, round cells in suspension; some cells are loosely adherent (detachable by shaking the flask)
Recommended Medium
90% DMEM + 10% horse serum. + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1 week post-thawing
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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