Description
Established in 1982 by immunizing BALB/c mice with VLM tumor cells and fusing splenocytes with SP2/0-Ag14 myeloma cells; described as secreting a mouse monoclonal antibody (IgG2akappa) detecting epitopes of wild type p53 (not mutant or denatured p53), that occur on human, primate, bovine, mouse and rat cells, by ELISA and immunoblotting
Karyotype
Murine flat-moded hypertetraploid karyotype - 82-97 - several centric fusion markers present
Source
hybridoma (anti-wild type p53)
Culture Properties
suspension
Morphology
single round cells growing in suspension, partly slightly adherent
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
