Established by fusion of NS-1 myeloma cells and spleen cells from a male BALB/c mouse immunized with SV40-transformed, 3T3 clone 4 mouse cells; cells were reported to secrete an IgG2b antibody recognizing p53 protein present in nuclei of SV40-transformed cells
single cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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