Described as secreting a mouse monoclonal antibody (IgG1) reacting with neonatal and adult slow and IIA fibers in rat and human and with slow and IIA fast myosin heavy chains in Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Cytogenetics: murine hypertetraploid karyotype with 8.3% polyploidy - 88(85-92) - carries 1-3 centric fusion markers, 1-3 dm
single round cells, lightly adherent
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assaysImmunology:secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.