Established from a stage III abdominal tumor of a 16-month-old girl with neuroblastoma; cells were described to contain two chromosomes with homogeneously stained regions (HSR′s) consisting of amplified MYCN (NMYC, N-myc) and MDM2 copies
polymorph cells growing adherently in monolayers
Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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