Description
Established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc)
Karyotype
Human bimodal hyper-, diploid/tetraploid karyotype - 54(48-54)<2n>XY, +1, +1, +2, +6, +7, +8, +13, +20, der(1)t(1;?)(p32;?)x2, der(13)t(13;?2HSR)(q33;?2p23) - both the long HSR on ch 13 due to MYCN (NMYC, N-myc) amplification and the ch 1 short-arm rearra
Culture Properties
monolayer
Morphology
adherent, fibroblast-like cells growing as monolayers
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: ELISA: reve
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
