Established from the primary adrenal tumor resected prior to treatment from a 3-year-old boy with rapidly progressing Stage III neuroblastoma in 1989; described to lack MYCN (NMYC, N-myc) amplification and chromosome 1p deletions, but to show prolonged N-myc protein half life
polymorph cells growing adherently in monolayers
Mycoplasma: contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofila
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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