Established from a bone marrow metastasis of a 6-year-old boy with neuroblastoma (clinical Stage IV)
loosely adherent, fibroblast-like cells growing as monolayers
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HT
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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