Established from the invasive transitional cell carcinoma of the bladder (grade 3, pT3b) of a 76-year-old man in 1993; described as secreting several hematopoietic cytokines and chorionic gonadotropin
Human hypertriploid karyotype - 74(70-75)<3n>XXX, +1, +5, +6, -7, +9, -11, +12, +15, +16, -17, -18, +19, +21, -22, +2mar - add(1)(p32-34)/del(1)(p34), add(4)(q35)x1-2, del(5)(q14.2), add(6)(q11), del(11)(p14), add(12)(q24)/der(12)t(12;17)(q24;q12-22), add
urinary bladder transitional cell carcinoma
epithelial-like cells growing adherently in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vim
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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Targeting AKT1-E17K and the PI3K/AKT pathway with an allosteric AKT inhibitor, ARQ 092
Authors: Yu Y, Savage R E, Eathiraj S, et al.
The PLoS One. 2015, 10(10): e0140479.