JMSU-1

Cat.No.: CSC-C0515

Species: Homo sapiens (Human)

Source: Ascites Metastasis

Morphology: adherent epitheloid cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0515

Description

Derived from malignant ascitic fluid of a 75-year-old man with urinary bladder carcinoma in 1988; described as being of epithelial origin with morphologically distinct cells (polygonal to spindle-shaped) and as expressing cytokeratin-19 fragment

Species

Homo sapiens (Human)

Source

Ascites Metastasis

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent epitheloid cells growing in monolayers

Karyotype

Human flat-moded hypertriploid karyotype with 12% polyploidy - 69-74<3n>X/XX, -Y, -Y, +1, +1, +2, -5, +9, -12, +13, -15, -15, -16, -17, +19, -22, +9mar, add(1)(q32), add(2)(q3?), del(4)(q31q33), del(6)(q25), add(7)(p11), del(8)(p22), del(9)(p22), del(10)(

Disease

Bladder Carcinoma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.