Description
Established in 1985 from the lymph node from a 42-year-old white man with metastatic small cell lung carcinoma prior to treatment; corresponds to NCI-H1184; matched B-lymphoblastoid cell line (B-LCL) is available
Karyotype
Human hyperdiploid karyotype with 8% polyploidy - 57(53-59)<2n>XY/XXY, +1, +3, +5, +8, +9, -10, -10, +11, +12, -14, +16, +20, +22, +6mar, add(1)(p34), add(1)(p11), add(3)(p14), add(3)(q21-22), add(5)(p11), add(5)(q11), add(8)(q21), add(11)(q25), add(12)(q
Source
small cell lung carcinoma
Culture Properties
monolayer
Morphology
small epitheloid cells growing adherently in monolayers or in suspension as clusters
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
