H-1184

Cat.No.: CSC-C0512

Species: Homo sapiens (Human)

Source: Lymph Node Metastasis

Morphology: small epitheloid cells growing adherently in monolayers or in suspension as clusters

Culture Properties: monolayer

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Cat.No.

CSC-C0512

Description

Established in 1985 from the lymph node from a 42-year-old white man with metastatic small cell lung carcinoma prior to treatment; corresponds to NCI-H1184; matched B-lymphoblastoid cell line (B-LCL) is available

Species

Homo sapiens (Human)

Source

Lymph Node Metastasis

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

monolayer

Morphology

small epitheloid cells growing adherently in monolayers or in suspension as clusters

Karyotype

Human hyperdiploid karyotype with 8% polyploidy - 57(53-59)<2n>XY/XXY, +1, +3, +5, +8, +9, -10, -10, +11, +12, -14, +16, +20, +22, +6mar, add(1)(p34), add(1)(p11), add(3)(p14), add(3)(q21-22), add(5)(p11), add(5)(q11), add(8)(q21), add(11)(q25), add(12)(q

Disease

Lung Small Cell Carcinoma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.