Derived from the lung of a 56-year-old black man with small cell lung carcinoma prior to treatment in 1988
Human bimodal hypodiploid/hypotetraploid aryotype with 3% polyploidy - 44(42-48)<2n>X, -Y, -5, -10, -13, +18, -19, +2mar, add(1)(q32), ins(2;hsr)(p21-23;?), add(3)(p11), del(4)(q24-26), del(10)(p13-14), add(18)(p11)x2, del(20)(q12), der(22)(t1;22)(q12;q13
small cell lung carcinoma
cells grow in clumps in suspension
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, G
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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