GMS-10

Cat.No.: CSC-C0450

Species: Homo sapiens (Human)

Source: Brain

Morphology: fibroblast-like cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0450

Description

Established from the malignant brain tumor of a 49-year-old man with glioblastoma in 1995

Species

Homo sapiens (Human)

Source

Brain

Recommended Medium

85-90% DMEM + 10-15% h.i.FBS

Culture Properties

monolayer

Morphology

fibroblast-like cells growing as monolayers

Karyotype

Human flat-moded hypertriploid karyotype with 11% polyploidy - 69-76<3n>XXYY, +Y, +2, +3, +9, -10, -12, +15, +15, -18, +20, +20, -22, +2-4mar, +1-4dmin, del(2)(p11), add(2)(q31;hsr), der(4)t(3;4)(p21;p15.3), add(4)(p1?), del(6)(q23), add(7)(q11), add(9)(p

Disease

Glioblastoma

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: rev

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.