CPC-N

Cat.No.: CSC-C0384

Species: Homo sapiens (Human)

Source: Pleural Effusion Metastasis

Morphology: mostly round cells growing in suspension in very large aggregates preventing exact estimation of cell numbers

Culture Properties: suspension

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Cat.No.

CSC-C0384

Description

Established from the pleural effusion metastasis of a patient with small cell lung carcinoma in 1987 (= CPN)

Species

Homo sapiens (Human)

Source

Pleural Effusion Metastasis

Recommended Medium

SuperCult® McCoys 5a Medium and 10% h.i. FBS.

Culture Properties

suspension

Morphology

mostly round cells growing in suspension in very large aggregates preventing exact estimation of cell numbers

Karyotype

Human hyperdiploid karyotype with 16% polyploidy - 49(47-51)<2n>XY, +6, +15, +20, add(1)(p36), i(3q), del(6)(q1?3q2?5), der(8)t(8;17)(p23;q21), add(13)(p11)del(13)(q12q22), add(15)(p12), ins(16;8)(p13;HSR?q24) - resembles published karyotype

Disease

Lung Small Cell Carcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin (+), cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

CPC.N; CPCN; CPN

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.