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COLO-679

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Cat.No.
CSC-C0363
Description
Established in 1985 from the soft tissue metastasis of a malignant melanoma from a 47-year-old woman
Species
Human
Source
melanoma
Recommended Medium
90-95% RPMI-1640 + 5-10% h.i. FBS
Morphology
adherent fibroblastic, but not limited to monolayer growth
Karyotype
Human, flat-moded, hypotetraploid karyotype with 8% polyploidy - 83-92<4n>XXXX, -1, +3, -4, +7, +7, -14, -15, -15, -16, -18, -20, -20, +3mar - del(3)(p11)x1-2, del(3)(q13.3), add(7)(q35)x2, del(9)(p22)x2, add(10)(p11)x1-2, add(10)(p13), add(14)(p11), add(
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament +, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, H
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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