Established in 1973 from an autopsy specimen of a 18-month-old boy with stage IV neuroblastoma after chemotherapy and irradiation treatment
polymorph cells growing adherently in monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament +, vimentin +
Viruses: PCR: EBV -, HBV -, HCV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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