CHP-126

Cat.No.: CSC-C0383

Species: Homo sapiens (Human)

Source: Retroperitoneal Space

Morphology: spheroidal cells growing as clumps in suspension; grows as semi-adherent monolayer on surfaces coated with poly-L-lysine

Culture Properties: suspension

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Cat.No.

CSC-C0383

Description

Established from a large retroperitoneal mass excised from a 14-month-old girl with neuroblastoma (stage III) in 1973; NMYC and DDX1 have been reported to be amplified

Species

Homo sapiens (Human)

Source

Retroperitoneal Space

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

spheroidal cells growing as clumps in suspension; grows as semi-adherent monolayer on surfaces coated with poly-L-lysine

Karyotype

Human near-diploid karyotype - 46(44-46)<2n>X, -X, +mar, del(1)(p36), t(2;14)(q37;q21-23), dup(7)(p15p22), der(11)t(1;11)(q22;q24), del(12)(q24.32), add(13)(p13), add(14)(p12) - ca. 20-50 dmin - del(1p32-36) and dmin (MYCN amp) are typical of late-stage n

Disease

Neuroblastoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.