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CHP-126

Cat.No.: CSC-C0383

Species: Human

Source: neuroblastoma

Morphology: spheroidal cells growing as clumps in suspension; grows as semi-adherent monolayer on surfaces coated with poly-L-lysine

Culture Properties: suspension

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Cat.No.
CSC-C0383
Description
Established from a large retroperitoneal mass excised from a 14-month-old girl with neuroblastoma (stage III) in 1973; NMYC and DDX1 have been reported to be amplified
Species
Human
Source
neuroblastoma
Recommended Medium
80% RPMI-1640 + 20% h.i. FBS
Culture Properties
suspension
Morphology
spheroidal cells growing as clumps in suspension; grows as semi-adherent monolayer on surfaces coated with poly-L-lysine
Karyotype
Human near-diploid karyotype - 46(44-46)<2n>X, -X, +mar, del(1)(p36), t(2;14)(q37;q21-23), dup(7)(p15p22), der(11)t(1;11)(q22;q24), del(12)(q24.32), add(13)(p13), add(14)(p12) - ca. 20-50 dmin - del(1p32-36) and dmin (MYCN amp) are typical of late-stage n
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are the advantages of DPBS buffer compared to PBS?

Compared to conventional PBS, DPBS does not contain Ca2+ and Mg2+ and there is no ionic residue when cells are washed with DPBS.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Satisfied

Creative Bioarray's cellular products meet the needs of research, and our laboratory will continue to purchase them.

27 Oct 2022


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