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CAT-13.9C1

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Cat.No.
CSC-C6155X
Description
Described as secreting a mouse monoclonal antibody (IgG2akappa) CD23; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed human B cell line from a patient with MHC class II deficiency re-expressing class II antigens
Organism
Mouse
Source
hybridoma (CD23)
Morphology
round cells, growing singly or in small clumps
Recommended Medium
90% DMEM + 10% h.i.FBS + HFCS ("Hybridoma Fusion and Cloning Supplement"for the first 1-2 weeks post-thawing)
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CM-1010X
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