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CAT-13.1E10

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Cat.No.
CSC-C6156X
Description
Described as secreting a mouse monoclonal antibody (IgG1) CD19; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with EBV-transformed B cells from a patient with MHC class-II deficiency re-expressing class-II antigens
Organism
Mouse
Source
hybridoma (CD19)
Culture Properties
suspension
Morphology
round cells growing as monolayers, some grow in suspension
Recommended Medium
80% DMEM + 20% h.i.FBS + HFCS ("Hybridoma Fusion and Cloning Supplement"for the first 1-2 weeks post-thawing)
Quality Control
Mycoplasma: contamination was eliminated with sparfloxacin, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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