Pseudohexadecaploid cell line generated by fusion of the 8-bromodeoxyuridine resistant mutant rat octaploid glioma cell line C6-3-BU-8 and the 6-thioguanine-resistant mutant rat octaploid glioma cell line C6-3-TG-3. C6-4-2 cells provide a suitable model system for studying the regulation of the membrane potential by peptide hormones.
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Liquid Nitrogen (-180 °C).
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