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BHT-101

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Cat.No.
CSC-C0373
Description
Established from the lymph node metastasis of a 63-year-old woman with anaplastic papillary thyroid carinoma; cells were described to not produce hormones, but to be partly positive for thyroglobulin- and thyroxine (T4)
Organism
Human
Karyotype
Apparently human hypertetraploid karyotype
Source
lymph node
Culture Properties
monolayer
Morphology
epithelial-like cells growing in monolayers
Recommended Medium
80% DMEM + 20% h.i.FBS + 0.5% human serum (+ 0.005 IU/ml TSH + 5 μg/ml human insulin - cells grow also without these latter supplements)
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP +, neurofilament -, thyr
Storage and Shipping
Frozen with 70% medium, 20% FBS, 0.5% human serum, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6254W
CSC-6255W
CSC-6309W
CSC-C0290
CSC-C0365
CSC-C0370
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