Established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described to contain parathyroid-like activity
Human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc.
epithelial-like cells growing as monolayer
Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neur
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.4-0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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