ActoFactor™ Recombinant Human Interleukin 8

CSC-CTK0380

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins which include NP-1, NP-2 and NP-3, are distinguished from the beta-defensins by the pairing of their three disulfide bonds. In addition to antimicrobial activity, NP-1 exhibits chemotactic activity on dendritic cells. NP-1 is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. NP-1 contains a six-cysteine motif that forms three intra-molecular disulfide bonds. Recombinant human NP-1 is a 3.4 kDa protein containing 30 amino acid residues.

Human

Human IL8 expressed in E.coli

3.4 kDa

CAT# CSC-CTK0380-20 (20 μg); CAT# CSC-CTK0380-100 (100 μg)

E.coli

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0- 10.0 ng/ml.

Less than 1 EU/μg.

Lyophilized (freeze-dried) powder.

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu NP-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.