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A4-951

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Cat.No.
CSC-C6162X
Description
Described as secreting a mouse monoclonal antibody (IgG1) reacting with adult slow fibers in rat and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin
Species
Mouse
Source
hybridoma (anti-fiber/myosin)
Recommended Medium
90% DMEM + 10% h.i.FBS + 1x non-essential amino acids
Culture Properties
suspension
Morphology
round cells, lightly adherent, growing singly and in clumps
Karyotype
Murine flat-moded hypertetraploid karyotype - 81(42-92)<4n> - 1-2 dmin and 1-2 centric fusion markers present in most cells
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CM-1010X

For research use only. Not for any other purpose.

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