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2A1

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Cat.No.
CSC-C6153X
Description
Established by fusion of mouse NS-1 cells with spleen from a BALB/c ByI mouse immunized with White Leghorn chicken embryonic retinal and tectal membranes; secretes 2A1 monoclonal antibody which binds epitopes involved in chicken retinal morphogenesis
Organism
Mouse
Karyotype
Murine hypertetraploid karyotype
Source
hybridoma (anti-chicken retina)
Culture Properties
suspension
Morphology
round cells growing singly in suspension or loosely adherent
Recommended Medium
80% RPMI-1640 I-1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
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Europe
Tel: 44-207-097-1828
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