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Immortalized Rat Portal Myofibroblasts (RGF)
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Cat.No.
CSC-I9263L
Description
Activated primary rat portal fibroblasts (PF) were immortalized with the SV40 large T antigen mammalian expression vector to generate the Immortalized Rat Portal Myofibroblasts (RGF). RGF cells are negative for hepatic stellate cell markers, and like the primary cells, they demonstrate inhibition of cholangiocyte proliferation in co-culture conditions. The easily transfectable nature of these cells make them an ideal research tool for studying both the function of myofibroblasts and their contribution to liver fibrosis progression.
Source
Liver
Culture Properties
Adherent
Morphology
Spindle
Immortalization Method
Serical passaging and chemical mediated transfection with SV40 large T Antigen mammalian expression vector
Markers
alpha smooth muscle actin, type I collagen alpha-1, tissue inhibitor of metalloproteinases-1, PF-specific markers elastin, type XV collagen alpha-1, Ntpdase2/Cd39l1, and ecto-5’-nucleotidase/Cd73
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Gene expression analysis to confirm the expression of common liver myofibroblast-associated genes.
2) Immunoblot and immunofluoresence microscopy to detect myofibroblast-specific cell markers.
3) Transfection assay to confirm DNA transfectability of cells.
4) Co-culture assay to demonstrate the inhibition of Mz-ChA-1 cholangiocarcinoma proliferation.
2) Immunoblot and immunofluoresence microscopy to detect myofibroblast-specific cell markers.
3) Transfection assay to confirm DNA transfectability of cells.
4) Co-culture assay to demonstrate the inhibition of Mz-ChA-1 cholangiocarcinoma proliferation.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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